Fig. 2
From: Bladder organoid conditioned media enhances myoblast proliferation under serum free conditions
Effect of mouse organoid-derived CM on the proliferation of C2C12 cells Representative bright-field microscopic images of C2C12 myoblasts cultured in different media (A). Top row: Serum-free control medium (left) and growth medium containing 10% FBS (right). Bottom row: Representative images of cells cultured in CM prepared by mixing organoid culture supernatants at a 1:1 ratio with low-glucose DMEM. Scale bars: 100 µm. Quantitative analysis of C2C12 cell proliferation based on fluorescence intensity (B). Data are expressed as mean ± SEM. n = 6 for biological replicates. *P < 0.05 vs. Control.
