Fig. 4
From: Bladder organoid conditioned media enhances myoblast proliferation under serum free conditions
Flow cytometric analysis of cell cycle progression in C2C12 cells cultured with MBOS. C2C12 myoblasts cultured under serum-free control conditions and MBOS were stained with propidium iodide (PI) to evaluate DNA content and cell cycle phase distribution using flow cytometry (A). Gates R2, R3, and R4 correspond to the G1, S, and G2/M phases, respectively. Quantification of cell populations in the G1 (R2), S (R3), and G2/M (R4) phases under control and MBOS conditions (B). Data are reported as mean ± SEM,n = 6,∗P < 0.05 vs. Control.
