Fig. 5
From: Bladder organoid conditioned media enhances myoblast proliferation under serum free conditions

Effect of growth factors in MBOS on C2C12 proliferation. The concentrations of insulin-like growth factors (IGF)1 and IGF2 were measured in serum-free control and MBOS-conditioned media using ELISA (A, n = 5). Data are reported as mean ± SEM.∗P < 0.05 vs. Control. Effect of IGF1 inhibition on MBOS-induced C2C12 cell proliferation. Neutralizing anti-IGF1 antibody (0.01–1 μg/mL) was added to the MBOS medium for 72 h, and cell proliferation was evaluated (B, n = 6). Data are reported as mean ± SEM. ∗P < 0.05 vs. Control.