Abstract
The increasing demand for sustainable agricultural practices has led to the exploration of beneficial microorganisms that promote plant growth and enhance resistance to phytopathogens. In this study, we characterized the Kol B9 strain isolated from the rhizosphere of Vinca minor. Morphological, biochemical, and molecular analyses were used to identify the strain as a member of the genus Bacillus, belonging to the Bacillus subtilis group. The strain exhibited strong antagonistic activity against Fusarium culmorum DSM 1094 and Fusarium sambucinum IM 6525, both on solid and in liquid media. This activity coincided with the production of surface-active cyclic lipopeptides and was accompanied by alterations in fungal membrane lipid composition, increased membrane permeability, and inhibition of spore germination and mycelial development. The environmental isolate F. sambucinum IM 6525 was less sensitive to Bacillus activity. In addition, B. subtilis Kol B9 promoted cucumber seedling growth and reduced the adverse effects of Fusarium infection. These findings support the potential application of B. subtilis Kol B9 as a bioinoculant in sustainable agriculture.
Data availability
The 16 S rRNA sequence of Bacillus sp. Kol B9 has been deposited in GenBank (NCBI) under accession number PX963729 and is publicly available at https://www.ncbi.nlm.nih.gov/nuccore/PX963729. Other datasets generated and/or analyzed during the current study are available from the corresponding author upon reasonable request.
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Acknowledgements
The experiments using microscopic techniques were carried out with the help of Łucja Balcerzak Ph.D. at the Laboratory of Microscopic Imaging and Specialized Biological Techniques of the Faculty of Biology and Environmental Protection, University of Lodz, Poland. We would like to thank Ms. Ewa Pawlicka for collecting soil samples from which the bacterial strains mentioned in this study were isolated.
Funding
This research was funded by the University of Lodz Doctoral School of Exact and Natural Sciences.
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A.J., A.W., K.G. wrote the main manuscript text. K.P, P.B, K.G., P.T. and A.W. advised on experimental design. A.J., A.W., K.G., P.T. prepared figures and tables; P.B., A.W., P.T., K.G., A.J. and P.B. conducted experiments. K.P., L.S.P, K.G., P.B. and A.J. supervised the study. All authors reviewed and approved the final manuscript.
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Jasińska, A., Walaszczyk, A., Bernat, P. et al. Biosurfactant-producing Bacillus spp. suppress Fusarium via fungal membrane disruption and promote cucumber growth. Sci Rep (2026). https://doi.org/10.1038/s41598-026-40391-z
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DOI: https://doi.org/10.1038/s41598-026-40391-z