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Imaging techniques

In vivo optical tissue clearing using light-absorbing dyes

Optical tissue clearing allows high-resolution microscopy imaging of biological samples while retaining critical three-dimensional and structural information for research and diagnosis. Most methods can only be performed ex vivo on fixed tissues, but a new study claims to have found a technique that can render live tissue transparent.

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Fig. 1: Relationship between the refractive index (RI) and light scattering.

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Correspondence to Maria Victoria Gómez-Gaviro.

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Gómez-Gaviro, M.V., Llorente, V. In vivo optical tissue clearing using light-absorbing dyes. Lab Anim 53, 361–362 (2024). https://doi.org/10.1038/s41684-024-01472-6

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