Optical tissue clearing allows high-resolution microscopy imaging of biological samples while retaining critical three-dimensional and structural information for research and diagnosis. Most methods can only be performed ex vivo on fixed tissues, but a new study claims to have found a technique that can render live tissue transparent.
This is a preview of subscription content, access via your institution
Access options

References
Rodriguez-Canales, J., Parra-Cuentas, E. & Wistuba, I. I. Cancer Treatment and Research (ed. Reckamp, K. L.) vol. 170 25–46 (Springer International Publishing, Cham, 2016).
Morgenstern, M. et al. Bone Jt. J. 100B, 966–972 (2018).
Sabino, R. & Wiederhold, N. J. Fungi 8, (2022).
Gómez-Gaviro, M. V., Sanderson, D., Ripoll, J. & Desco, M. iScience 23, 101432 (2020).
Susaki, E. A. et al. Nat. Protoc. 10, 1709–1727 (2015).
Cai, R. et al. Nat. Protoc. 18, 1197–1242 (2023).
Mai, H. et al. Nat. Biotechnol. 42, 617–627 (2024).
Ou, Z. et al. Science 385, eadm6869 (2024).
Costantini, I. et al. Biomed. Opt. Express 10, 5251 (2019).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Rights and permissions
About this article
Cite this article
Gómez-Gaviro, M.V., Llorente, V. In vivo optical tissue clearing using light-absorbing dyes. Lab Anim 53, 361–362 (2024). https://doi.org/10.1038/s41684-024-01472-6
Published:
Version of record:
Issue date:
DOI: https://doi.org/10.1038/s41684-024-01472-6