Fig. 3: Effect of PDHA1 on changes in biological functions of CRC Cells. | npj Precision Oncology

Fig. 3: Effect of PDHA1 on changes in biological functions of CRC Cells.

From: RNF4 mediated degradation of PDHA1 promotes colorectal cancer metabolism and metastasis

Fig. 3

A Western blot analysis of PDHA1 protein expression in various CRC cell lines and human normal colon mucosal cells (FHC). * indicates difference compared to the FHC group, P < 0.05; B Western blot analysis of PDHA1 protein expression in cells after overexpression or knockdown of PDHA1; C CCK-8 assay measuring changes in cell viability after overexpression or knockdown of PDHA1 at 24, 48, and 72 h; D Scratch assays measuring cell migration after overexpression or knockdown of PDHA1 (scale bar = 100 μm); E Transwell assay measuring cell migration and invasion after overexpression or knockdown of PDHA1 (scale bar = 50 μm); F RT-qPCR analysis of changes in mRNA levels of Snail, Slug, Twist, Vimentin, and E-cadherin after overexpression or knockdown of PDHA1. * indicates difference compared to the pcDNA group, P < 0.05; # indicates difference compared to the sh-NC group, P < 0.05. Cell experiments were repeated three times.

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