Fig. 6: The Influence of RNF4 on the biological functions of CRC cells by regulating PDHA1 protein.
From: RNF4 mediated degradation of PDHA1 promotes colorectal cancer metabolism and metastasis
A RT-qPCR detection of RNF4 and PDHA1 mRNA expression in SW620 cells from each group; B Western blot detection of RNF4 and PDHA1 protein expression in SW620 cells from each group; C CCK-8 assay to measure the viability changes of SW620 cells from each group at 24, 48, and 72 h; D Transwell assay to measure the migration and invasion ability of SW620 cells from each group (scale bar = 50 μm); E Scratch assay to measure the migration of SW620 cells from each group (scale bar = 100 μm); F RT-qPCR detection of Snail, Slug, Twist, Vimentin, and E-cad mRNA levels in SW620 cells from each group. * indicates P < 0.05 compared to sh-NC group, # indicates P < 0.05 compared to sh-RNF4 group, & indicates P < 0.05 compared to the sh- PDHA1 group. The experiments were repeated three times.
