Fig. 7: The regulation of RNF4/PDHA1 axis on CRC cell glycolysis level.
From: RNF4 mediated degradation of PDHA1 promotes colorectal cancer metabolism and metastasis
A Volcano plots showing the differential metabolites between the m-CRC and p-CRC groups, p-CRC: n = 26, m-CRC: n = 28; B Pathway enrichment analysis of differential metabolites using the MetaboAnalyst database; C Function enrichment analysis of differential metabolites using the MetaboAnalyst database; D, E Extracellular acid ratio (ECAR) and oxygen consumption rate (OCR) of cells overexpressing or knockdown of PDHA1 measured using Seahorse XF; 2-DG: 2-deoxyglucose, FCCP: Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone; F Lactate production in cells overexpressing or knockdown of PDHA1; G, H Extracellular acid ratio (ECAR) and oxygen consumption rate (OCR) of SW620 cells from each group measured using Seahorse XF; I Lactate production in SW620 cells from each group. In D–F, * indicates P < 0.05 compared to the pcDNA group, # indicates P < 0.05 compared to sh-NC group; in G–I, * indicates P < 0.05 compared to sh-NC group, # indicates P < 0.05 compared to sh-RNF4 group. The experiments were repeated three times.
