Fig. 4: Cell communications between YTHDC2-high/YTHDC2-low macrophages and other immune cells.

a Circle plot visualizing the communication strength between YTHDC2-low (upper panel)/YTHDC2-high (lower panel) macrophages and other immune cells in all samples composed of both the Chemo cohort and the Chemo + Anti-PDL1 cohort. The thickness of the line indicates the strength of intracellular communications. b Violin plots showing the normalized scRNA-seq expression distribution of selected ligand-receptor pairs for YTHDC2-low and YTHDC2-high tumor macrophage samples. c Expression of receptor-ligand pairs that can mediate interaction between tumor macrophage clusters YTHDC2-high/YTHDC2-low and other immune cell clusters, ligand from macrophages, and the receptor on other immune cells (CD4⁺ T_EM: CD4⁺ effector memory T cell; CD4⁺ T_REG: CD4⁺ regulatory T cell; CD8⁺ T_EM: CD8⁺ effector memory T cell; CD8⁺ T_RM: CD8⁺ resident memory T cell; CD8⁺ T_N: CD8⁺ naive T cell; NK: natural killer cell) in the Chemo cohort (upper panel) and the Chemo + Anti-PDL1 cohort (lower panel). Dot color reflects communication probabilities and dot size represents computed p-values (one-sided permutation test) for interactions that increased in YTHDC2-high or YTHDC2-low macrophages; empty space indicates no difference between YTHDC2-high and YTHDC2-low macrophages. Red boxes indicate the YTHDC2-high/YTHDC2-low change in the signals derived from MHC class I molecules and MHC class II molecules.