Fig. 1: Quality controls of cfRNA sequencing data.

a Schematic diagram showing study design workflow. b, c The ratio of clean reads that aligned to (b) tsRNA or (c) miRNA of different groups. The extremes of boxes define the upper and lower quartiles, and the center lines define the median. Whiskers indicate 1.5× interquartile range. d Principal component analysis on each group of samples using the top 20% of cfRNAs, ranked by their expression levels. e Transcriptome-wide Pearson’s correlation coefficients of the normalized sequencing reads of different types of RNAs between different groups.