Fig. 2: Lestaurtinib induces cell cycle arrest, apoptosis, and prevents colony formation. | npj Precision Oncology

Fig. 2: Lestaurtinib induces cell cycle arrest, apoptosis, and prevents colony formation.

From: Lestaurtinib’s antineoplastic activity converges on JAK/STAT signaling to inhibit treatment naïve and therapy resistant forms ovarian cancer

Fig. 2

a Indicated cell lines expressing a fluorescent cell cycle reporter were treated with multiple concentrations of lestaurtinib for 24 h and cell cycle phase distribution was monitored. The percentage of cells in each phase of the cell cycle was quantified via the IncuCyte S3 system based on the presence of nuclear red (G1), nuclear green (G2/M) and nuclear yellow (S). Representative images of the ABTR#2 cell line are shown. N = 5–8. Graphs represent mean ± standard error. ANOVA p-values compared to DMSO for each cell cycle stage: *<0.0332. b Nuc Red expressing cells were incubated with Caspase 3/7-GFP or Annexin V-GFP reporters and subsequently treated with lestaurtinib for 2 days. Percent apoptotic cells were defined as # green positive cells relative to # of red positive cells. Representative images of the ABTR#2 cell line are shown. N = 3–5. c Colony formation was assessed in the indicated cell lines following 3 weeks of treatment with multiple concentrations of lestaurtinib and the number of colonies were quantified by crystal violet staining. N = 3. Representative images of the ABTR#2 cell line are shown. Abbreviations: Nuc nuclear. Graphs represent mean ± standard error. ANOVA p-values: *<0.0332,**<0.0021,***<0.0002,****<0.0001.

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