Fig. 6: Lestaurtinib synergizes with cisplatin and olaparib.

Indicated cells were treated with cisplatin (25,000 nM or 10,000 nM, threefold dilution) or olaparib (25000 nM, threefold dilution), alone and in combination with lestaurtinib (5000 nM or 1000 nM, threefold dilution), for 5–7 days. Relative cell viability was measured with crystal violet staining. a, c Synergy plots depicting Loewe scores generated using SynergyFinder3.0. b, d Combination index (CI) scores were calculated using the Chou-Talalay method and Compusyn software. CI-ED50 = combination index at 50% fraction effected, CI-ED95 = combination index at 95% fraction effected, where the fraction effected represents precent cell death. Antagonism = >1 (black), additive = 1 (gray), synergy = < 1 (blue), and highly synergistic = < 0.3 (red). Pink highlighting = synergism identified using clinically achievable concentrations of both drugs.