Fig. 8: Lestaurtinib inhibits ERK and JNK leading to blockade of STAT S727 phosphorylation.

a Venn diagram of kinases known to be inhibited by lestaurtinib and predicted to phosphorylate S727 of STAT1 and/or STAT3. Western blots depicting total and phosphorylated levels of STAT1, STAT3, ERK and JNK following 30 min of (b) lestaurtinib, c LY3214996 (ERKi) or d CC-90001 (JNKi) treatment. e Western blots depicting total and phosphorylated levels of cJUN following 30 mins of lestaurtinib, LY3214996 (ERKi), or CC-90001 (JNKi) treatment. Vinculin served as the loading control.