Fig. 8: In CRC, ARHGAP4 promotes the increase of c-Jun to form a positive feedback loop.
From: ARHGAP4/MYH9/β-catenin/c-Jun positive feedback loop promotes colorectal cancer stemness
A and B The mRNA levels of c-Jun and MYH9 in RKO and HCT116 cells; C and D The protein changes of MYH9, β-catenin and c-Jun; E and F WB was used to detect the changes of c-Jun and MYH9 protein levels in the case of pathway recovery; G Venn diagram of the intersection of ARHGAP4 upstream transcription factors in GTRD database and Knock TF database; H JASPR database showed the sites with the highest binding score in the c-Jun and ARHGAP4 promoter regions; I The correlation between ARHGAP4 and c-Jun was analyzed by TCGA database; J ChIP analysis verified that c-Jun could interact with the promoter of ARHGAP4; K c-Jun transcriptional regulation of ARHGAP4 promoter activity report; L the mRNA levels of ARHGAP4 and MYH9 under the change of c-Jun expression; M The protein levels of ARHGAP4 and MYH9 were changed under the change of c-Jun expression.
