Fig. 8: Stable HDM2 overexpression differentially modulates gene expression in human NSCLC cells with different TP53 genotypes, mimicking patterns seen in NACC2 mutant cells.
From: LINE1 insertion into the NACC2 locus disrupts the HDM2 axis and activates lung oncogenic signaling
a HDM2 overexpression was achieved by lentivirus-mediated transduction. Viral particles were collected from cells transfected with the HDM2 plasmid, while a vector plasmid lacking the HDM2 sequence was used as a control. b–e EGFR fluorescence of individual cells transfected with the pWPXL-HDM2 plasmid was monitored after lentivirus transfection. f Representative Western blots for HDM2, ORF1p, p53, and RB. Beta-actin was used as a loading control. N = 3.
