Fig. 1: Diagram of analytic workflow. | npj Precision Oncology

Fig. 1: Diagram of analytic workflow.

From: Integrative bioinformatics analysis and experimental validation identify CHEK1 as an unfavorable prognostic biomarker related to immunosuppressive phenotypes in soft tissue sarcomas

Fig. 1

Schematic overview of the integrative analysis pipeline used in this study. Transcriptomic, proteomic, and clinical data were collected from public databases (GEO and TCGA) and an RMS cohort. Differential expression analyses and immune deconvolution (CIBERSORTx) were performed to estimate tumor-infiltrating immune cell populations. Unsupervised clustering based on immune cell subsets defined distinct immune phenotypes. CHEK1 was identified as a candidate biomarker associated with immunosuppressive phenotypes and poor prognosis. Validation was conducted via immunohistochemistry (IHC), multiplex immunohistochemistry (mIHC), and survival analyses in independent cohorts.

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