Extended Data Fig. 5: Amplicon sequencing results of target-distal edits. | Nature Catalysis

Extended Data Fig. 5: Amplicon sequencing results of target-distal edits.

From: ProPE expands the prime editing window and enhances gene editing efficiency where prime editing is inefficient

Extended Data Fig. 5

a,b, PE5max/proPE5max (a) and SpCas9 nuclease (b) editing on 3 genomic targets using tpg/pegRNA with a tevopreQ1 extension. ‘eng fractions’ refers to different amounts of engRNA plasmid. c-g, Data are from the same experiment as shown in Fig. 6b, c. All information is provided in the main figure. Data are presented as mean values (a-g) ± SD (a,b,e-g). The dashed line shows the 0.3% threshold value (f).

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