Extended Data Fig. 6: Editing various cell lines. | Nature Catalysis

Extended Data Fig. 6: Editing various cell lines.

From: ProPE expands the prime editing window and enhances gene editing efficiency where prime editing is inefficient

Extended Data Fig. 6

Amplicon sequencing results of PE5max and the corresponding proPE5max editing on the 8 genomic targets (shown also in Fig. 6d, e) in HEK293, U2OS and K562 cell lines using tpg/pegRNAs with tevopreQ1 extension. In a, the editing efficiency, b, the indels formed and c, specificity are shown. Note the logarithmic scale of the ordinate in b and c. For each modification, three engRNA fractions were used. ProPE caused an increase in editing efficiency (a) and specificity (c) in all three cell lines. The indel forming effect of proPE decreased with lower engRNA fractions (b). Data are presented as mean values ± SD. The dashed line shows the 0.3% threshold value (b).

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