Extended Data Fig. 7: Amplicon sequencing results. | Nature Catalysis

Extended Data Fig. 7: Amplicon sequencing results.

From: ProPE expands the prime editing window and enhances gene editing efficiency where prime editing is inefficient

Extended Data Fig. 7

PE4max/proPE4max (d-g) and PE5max/proPE5max (a-g) editing results are shown using tpg/pegRNAs with tevopreQ1 extension (a-g). Data in panels a-c supplement Fig. 6f,g. d,e, ProPE increases the editing efficiency (d) and specificity (e) for both PE4max (N = 12) and PE5max (N = 19) editing types using 13-nt-long PBSs in the CYP genes. f,g, ProPE has a strong enhancing effect on editing efficiency and specificity on low-performing edits on the CYP genes. (N = 53). Data are presented as mean values (a-g) ± SD (a-c). The dashed line shows the 0.3% threshold value (b). Medians are shown (d-g), statistical significance was assessed by a two-tailed Mann-Whitney test (d-f), p-values and fold change between proPE and PE are indicated on the top and next to the dots, respectively (d-f). See Extended Data Fig. 10 for further details of the data in d-g.

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