Extended Data Fig. 9: ProPE appears to outperform other split pegRNA approaches and is compatible with AAV-based delivery. | Nature Catalysis

Extended Data Fig. 9: ProPE appears to outperform other split pegRNA approaches and is compatible with AAV-based delivery.

From: ProPE expands the prime editing window and enhances gene editing efficiency where prime editing is inefficient

Extended Data Fig. 9

Amplicon sequencing results of prime editing using the tevopreQ1 extension except for sPE-petRNA (a,b) for which a circular RNA is used. a,b, Comparison of the activity of split PE with petRNA23 and two SnPE variants37 (SnPE-5’-MS2, SnPE-5’-BoxB) with proPE and PE on nine edits using the PE4max/PE5max system, presented as aggregates (a) and as individual edits (b), using an identical amount of nicking sgRNA coding plasmid for the corresponding PE variants. ProPE shows a consistently higher activity than the other PE variants. c, ProPE is compatible with AAV delivery. Comparison of proPE delivery systems of plasmid transfection with either our regular conditions or with AAV plasmids and AAV transduction. The results of the regular plasmid transfection from Extended Data Fig. 4a were used as a reference here. Statistical significance was assessed by a Kruskal-Wallis test with two-tailed Dunn’s test (a). Data (a-c) are presented as mean values ± SD (b,c).

Source data

Back to article page