Extended Data Fig. 10: Gene-specific editing can be achieved by proPE.
Amplicon sequencing results of PE and proPE using the tevopreQ1 extension. We present the results of two approaches for efficient gene-specific proPE editing, still applicable when PE fails due to the large distance between the desired modification and sequence variations in homologous genes that are amenable to targeting. The actual layouts for these edits and targets are presented in Fig. 8c. Although a higher level of editing can be achieved using proPE5max the gene specificity in terms of the ratio of on-target and off-target edit is not improved compared to proPE4max, while proPE5max produces a significantly higher background of on- and off-target indels. All data are presented as mean values ± SD. Data supplement Fig. 8d, e.
