Fig. 6: The summary for context-dependent DSB repair processing of SSA-based transgenes at Ae. aegypti kmo. | Communications Biology

Fig. 6: The summary for context-dependent DSB repair processing of SSA-based transgenes at Ae. aegypti kmo.

From: Transgene removal using an in cis programmed homing endonuclease via single-strand annealing in the mosquito Aedes aegypti

Fig. 6

Transgenic mosquito strains were generated to contain various lengths of DR motifs (0.2-kb, 0.7-kb, or 1.5-kb) and the intervening cargo genes (3.7-kb or 7.9-kb), and their DNA repair dynamics were evaluated by crossing DSB trigger strains that express homing endonucleases (I-SceI or I-AniI) under the control of germline-specific nos promoter. One-piece SSA-featured mosquitoes were crossed with the kmo−/− strain in DNA repair tests. The [R] analysis presents the proportional values of cargo lengths (bp) divided by direct repeat lengths (bp) with respect to eliminating efficiency of kmo-integrated transgenes. Selection biases of DNA repair pathways were shown by the proportional values of SSA rates (%) normalized by NHEJ rates (%).

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