Fig. 7: Ichthyosporea 18S rRNA gene phylogeny highlights uncultured diversity.

Maximum-likelihood phylogeny of 315 unique Ichthyosporea 18S rRNA genes (and 108 outgroup sequences) collected from various data sources (see Methods and Supplementary Data 4) inferred using the GTR + FO + R7 model of evolution (alignment length: 1800 nt). Supported and newly described clades are collapsed and those newly identified are indicated in pink. Sequences within each clade were assigned to environment categories according to EukBank96 criteria as outlined in the Fig. 6 legend, and are indicated by circles according to the legend (land_water was excluded). A star next to a clade indicates that it includes a cultured representative. Support from ultrafast bootstraps (ufboot) and non-parametric bootstraps (BP) is indicated by circles according to the legend. Branches with less than 90% ufboot support are collapsed. See Supplementary Data 5 and 6 for alignments and uncollapsed trees and for a maximum-likelihood phylogeny including only long (≥1000 nt) 18S rRNA genes from species representatives (97% sequence identity).