Fig. 3: Afucosylated anti-HIV-1 bNAbs induce ADCC of HIV-1 infected cells.

a ADCC activity of CEM.NKr.CCR5-luc infected cells in the presence of a serial dilution of 2G12 (red; circles) and N6 variants (blue; triangles). Mean + standard deviation of triplicate measurements are plotted, where fucosylated bNAbs are displayed with a closed symbol and afucosylated bNAbs with an open symbol. b Bar graph showing mean ADCC activity + standard deviation of the fucosylated (dark gray) and afucosylated (light gray) Abs at a concentration of 10 μg/ml. Each dot represents the ADCC activity (mean of triplo in Supplementary Fig. 4b) measured against BG505.T332N, LAI or AMC016 infected CEM cells using PBMCs from three different donors. Thus, this figure combines all data plotted in the nine individual graphs in Supplementary Fig. 4b. Percentage ADCC is derived from the raw luciferase data using the following formula: Luciferase count in the presence of effector cells alone minus luciferase count in the presence of antibody and effector cells, divided by luciferase count in the absence of antibody and effector cells, multiplied by 100. Statistical differences between fucosylated and afucosylated bNAbs were determined using an ordinary one way Anova with a Šídák's multiple comparisons test and significant differences are indicated with asterisks. *p < 0.05; **p < 0.01; ***p < 0.001.