Fig. 4: Afucosylated anti-HIV-1 bNAbs induce ADCC of latently infected cells. | Communications Biology

Fig. 4: Afucosylated anti-HIV-1 bNAbs induce ADCC of latently infected cells.

From: Afucosylated broadly neutralizing antibodies enhance clearance of HIV-1 infected cells through cell-mediated killing

Fig. 4

a Percentage p24+ (black line, circles) and Env+ (red line, squares) ACH-2 cells are plotted after reactivation with various concentrations of TNFα. The adjacent flow cytometry dot plots show Env+ and p24+ cells in unstimulated and TNFα (10 ng/ml) reactivated ACH-2 cells. b Quantification of target cell (ACH-2) killing and effector cell (PBMC) activation using a flow cytometry based ADCC assay for which the gating strategy is described in Supplementary Fig. 5b. Mean + standard deviation of triplicate measurements are plotted for ADCC of ACH-2 cells (top panel), PBMC activation (CD69+CD107+ PBMCs, middle panel) and CD16 shedding (bottom panel) in the presence of 10 µg/ml fucosylated (dark gray) or afucosylated antibody (light gray).

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