Fig. 3: Characterization of the effects of human platelet- and red blood cell-EVs on lymphatic endothelial cells in vitro. | Communications Biology

Fig. 3: Characterization of the effects of human platelet- and red blood cell-EVs on lymphatic endothelial cells in vitro.

From: Platelet extracellular vesicles preserve lymphatic endothelial cell integrity and enhance lymphatic vessel function

Fig. 3

PEVs stained with CellVue (red) were incubated on LEC in vitro for (a) 2 h and (b) 24 h and incubated with WGA (green) and Hoechst (blue). Z-stacks were acquired using a LSM 710 Confocal Microscope (Zeiss) equipped with a 63/1.4 oil DIC objective (white scale bar, 20 µm). (c) rbEVs, (d) PEVs and LECs treated with (e) rbEVs or (f) PEVs for 24 h were incubated with CM-H2DCFDA and ROS production was measured using flow cytometry. Superoxide dismutase (100 U/mL) was used as a negative control. The percentage of AnnexinV-Propidium iodide+ (PI+AnV-) LECs was determined after treatment with (g) PEVs or (h) rbEVs. The concentration of EVs produced from LECs in the culture supernatant was measured after treatment with (i) rbEVs or (j) PEVs. Each point (n) represents cells treated with PEVs produced from a single donor ± SEM. PEVs: platelet extracellular vesicles; rbEVs: red blood cell extracellular vesicles; WGA: Wheat Germ Agglutinin; ROS: reactive oxygen species; SOD: superoxide dismutase. AU: arbitrary unit.

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