Fig. 2: PBHL-controlled uncages DNA on glass slides.
From: Large field of view and spatial region of interest transcriptomics in fixed tissue
A Schematic for PBHL-directed barcode attachment on glass slides. Amino-modified single-stranded poly A oligos are immobilized onto Codelink glass surfaces and NPOM-caged oligodeoxynucleotides are hybridized. Target pixels corresponding to ROIs in the field of view are photo-illuminated using the mosaic system by the selected pattern. Fluorescent barcode strands are hybridized to the uncaged barcode oligos. Unhybridized strands are removed by washes, which reveals the illuminated area with fluorescence. B Iterative photo-irradiation using two geometry that defines two ROIs to attach two pattern barcode strands onto a DNA-coated glass slide. Scale bar, 50 μm.
