Fig. 4: Application of PBHL-seq for spatial barcoding in fixed frozen mouse brain sections.
From: Large field of view and spatial region of interest transcriptomics in fixed tissue
A Four regions of the mouse brain are uniquely barcoded for sequencing. Scale bar, 1000 µm. B, C Boxplot of numbers of detected genes (B, n = 4 biological replicate) and Unit-area UMIs for each ROI (C, n = 4 biological replicate). Midline marks the median and edges indicate the 25th and 75th percentiles, and the x mark represents the average value. D Two-dimensional principal component analysis (PCA) for the expression profiling is shown. E Volcano plots of differentially expressed genes, with selecting markers labeled. The x and y axes show the log2(fold-change) and the −log10(P-value), respectively.
