Fig. 5: Application of PBHL-seq for spatial barcoding in FFPE mouse embryo sections.
From: Large field of view and spatial region of interest transcriptomics in fixed tissue
A Two ROIs on the mouse embryo section, the eye, and viscus, are uniquely barcoded with PBHL-seq. Scale bar, 500 μm. B, C Boxplot of numbers of detected genes (B, n = 3 biological replicate) and Unit-area UMIs (C, n = 3 biological replicate). The midline marks the median and the edges indicate the 25th and 75th percentiles, and the x mark represents the average value. D Two-dimensional PCA for ROI expression profiling is shown. E Heatmap of twenty-two genes with known differential expression between eye and viscus (P-value  <  0.05 and |log2(fold-change)| > 1 are shown, n  =  3 biological replicate). E Volcano plots of differentially expressed genes between the eye and viscus, with only a few better characteristic marker genes labeled. The x and y axes show the log2(fold-change) and the log10(P-value), respectively.
