Fig. 6: PBHL-seq for subcellular compartments.
From: Large field of view and spatial region of interest transcriptomics in fixed tissue
A Two regions of Hela cells are uniquely fluorescent barcoded with ROX and FAM dye primers. Scale bar, 25 μm. B Images of green immunofluorescence (IF) for cytoplasm and red DRAQ5 for nucleus. The UV irradiation part region is shown with cycles. Scale bar, 10 μm. C, D Boxplot of numbers of detected genes (C, n = 4 biological replicate) and Unit-area UMIs (D, n = 4 biological replicate). Midline marks the median and edges indicate the 25th and 75th percentiles, and the x mark represents the average value. E Two-dimensional PCA for the expression profiling is shown. F Volcano plots of differentially expressed genes between the nucleus and cytoplasm, with selecting markers labeled. The x and y axes show the log (fold-change) and the -log10 (P-value), respectively.
