Fig. 7: Application of PBHL-seq for a small number of cells transcriptomics in tissue.
From: Large field of view and spatial region of interest transcriptomics in fixed tissue
A Two ROIs on the mouse embryo section, retinal pigment epithelial (RPE) cells (show part photo-illuminate cells) and neural retina (NR), are uniquely barcoded with PBHL-seq. The scale bar of the mouse embryo is 500 μm. In particular, the scale bar of zoomed-in images is 25 μm. B, C Boxplot of numbers of detected genes (B, n = 4 biological replicate) and Unit-area UMIs (C, n = 4 biological replicate). The midline marks the median and edges indicate the 25th and 75th percentiles, and the x mark represents the average value. D The correction of different replications (n = 4 biological replicate). E Two-dimensional PCA for ROI expression profiling is shown. F Volcano plots of differentially expressed genes between the eye and viscus, with only a few better characteristic marker genes labeled. The x and y axes show the log2(fold-change) and the log10(P-value), respectively.
