Fig. 4: FMO3 knockout suppresses ER stress at the acute phase after renal I/R. | Communications Biology

Fig. 4: FMO3 knockout suppresses ER stress at the acute phase after renal I/R.

From: Deficiency of flavin-containing monooxygenase 3 protects kidney function after ischemia–reperfusion in mice

Fig. 4

a RT-qPCR results showing mRNA expression of ER-Stress related genes Atf4, Ddit3, Xbp1, Ern1 detected in WT and Fmo3−/− kidney tissues 48 h after I/R. All normalized to Gapdh, n = 5 per group. *P < 0.05; **P < 0.01; ***P < 0.001 (one-way ANOVA with post hoc Tukey test). b Representative immunoblotting images showing protein levels of p-PERK, PERK, p-eIF2α, eIF2α, ATF4, CHOP, XBP1s, ATF6, and HSP90 in WT Sham, Fmo3−/− Sham, WT I/R and Fmo3−/− I/R kidney tissues (left panel). Quantification of protein levels of p-PERK and p-eIF2α are normalized by PERK and eIF2α, respectively (right panel). Quantification of protein levels of ATF4, CHOP, XBP1s, and ATF6 are normalized by HSP90 (right panel), n = 3 per group. ns not significant; *P < 0.05; **P < 0.01 (one-way ANOVA with post hoc Tukey test).

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