Table 1 Efficiency of derivation of PGCs and established PGC lines from four Chinese chicken populations (preliminary experiment)

From: PGC-based cryobanking, regeneration through germline chimera mating, and CRISPR/Cas9-mediated TYRP1 modification in indigenous Chinese chickens

Breed (population)

No. of collected eggs

No. of fertilized eggs (%)

No. of embryonic blood samples collected

No. of established PGC lines (derivation efficiency %)

Average cell countsa

Average no. of culture daysb

Average survival rate of cells at cryopreservation (%)

Total

Male

Female

Total

Male

Female

Xichou black bone

30

24 (80.0%)

24

15

9

23 (95.8%)

14 (93.3%)

9 (100%)

3.3 × 105

33.7

89.7%

Chahua (YAU)

35

30 (85.7%)

25

9

16

14 (56%)

8 (88.9%)

6 (37.5%)

4.9 × 105

26.1

82.5%

Wuding

10

7 (70.0%)

6

2

4

3 (50%)

2 (100%)

1 (25.0%)

2.5 × 105

44.3

73.7%

Crossbreed (PCM)

8

6 (75.0%)

6

4

2

5 (83.3%)

4 (100%)

1 (50.0%)

4.7 × 105

25.6

91.4%

Total (♂・♀)

83

67

61

-

-

45 (73.8%)

-

-

3.9 × 105

31.2

86.6%

Total (♂)

-

-

-

30

-

-

28 (93.3%)

-

4.1 × 105

25.7

86.9%

Total (♀)

-

-

-

-

31

-

-

17 (54.8%)

3.5 × 105

40.2

86.0%

  1. a, bEach PGC line was cryopreserved after several days, following confirmation that the cell count exceeded 1 × 105. On the day of cryopreservation, the cell count and cell viability were counted again, and the cells were divided into multiple vials to ensure a number of 1 × 105 cells per vial. The cell count represents the number of cells on the day of cryopreservation, while the culture days indicate the number of days until the cryopreservation was carried out. The average cell count and average culture days represent the mean values within each group. The total numbers and ratios for established PGC lines of males, females, and both male and female specimens were indicated by bold letters.
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