Table 2 Efficiency of derivation of PGCs and established PGC lines from four heritage populations of two Chinese breeds, Chahua and Piao (cryobanking)
Breed (Population) | Date of egg recept | No. of collected eggs | No. of fertilized eggs (%) | No. of embryonic blood samples collected | No. of established PGC lines (derivation efficiency %) | Total no. of expanded cells (average) | No. of culture days (average) | Survaival rate of cells at cryopreservation(average) | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Total | Male | Female | Total | Male | Female | |||||||
Chahua (A) | 2020-08-11 | 20 | 8 | 4 | 4 | 0 | 4 (100%) | 4 (100%) | 0 | 2.89 × 105 ♂: 3.18 × 105 ♀: 2.19 × 105 | 24.3 ♂: 22.2, ♀: 29.3 | 90.6% ♂: 90.5%, ♀: 90.8% |
2020-09-08 | 30 | 29 | 25 | 17 | 8 | 23 (92.0%) | 17 (100%) | 6 (75%) | ||||
2020-10-13 | 10 | 9 | 7 | 4 | 3 | 6 (85.7%) | 4 (100%) | 2 (67%) | ||||
2021-02-01 | 60 | 47 | 35 | 21 | 14 | 32 (91.4%) | 21 (100%) | 11 (79%) | ||||
Total | 120 | 93 (77.5%) | 71 | 46 | 25 | 65 (91.5%) | 46 (100%) | 19 (76.0%) | ||||
Chahua (C) | 2020-08-11 | 20 | 5 | 4 | 2 | 2 | 4 (100%) | 2 (100%) | 2 (100%) | 2.93 × 105 ♂: 3.04 × 105 ♀: 2.63 × 105 | 25.7 ♂: 24.2, ♀: 29.7 | 87.3% ♂: 87.3%, ♀: 87.1% |
2020-09-08 | 30 | 29 | 26 | 15 | 11 | 23 (88.5%) | 15 (100%) | 8 (73%) | ||||
2020-10-13 | 10 | 10 | 8 | 7 | 1 | 8 (100%) | 7 (100%) | 1 (100%) | ||||
2021-03-29 | 60 | 57 | 39 | 24 | 15 | 24 (61.5%) | 19 (79%) | 5 (33%) | ||||
Total | 120 | 101 (84.2%) | 77 | 48 | 29 | 59 (76.6%) | 43 (89.6%) | 16 (55.2%) | ||||
Chahua (D) | 2020-08-11 | 20 | 7 | 6 | 4 | 2 | 6 (100%) | 4 (100%) | 2 (100%) | 3.3 × 105 ♂: 3.7 × 105 ♀: 2.4 × 105 | 25.5 ♂: 24.7, ♀: 27.4 | 88.9% ♂: 89.2%, ♀: 88.1% |
2020-09-08 | 30 | 29 | 17 | 9 | 8 | 17 (100%) | 9 (100%) | 8 (100%) | ||||
2020-10-13 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||
2021-06-22 | 60 | 60 | 40 | 26 | 14 | 29 (72.5%) | 23 (88%) | 6 (43%) | ||||
Total | 120 | 96 (80%) | 63 | 39 | 24 | 52 (82.5%) | 36 (92.3%) | 16 (66.7%) | ||||
Chahua (A, C, D) | Total | 360 | 290 (80.5%) | 211 | 133 | 78 | 176 (83.4%) | 125 (94.0%) | 51 (65.4%) | 3.0 × 105 ♂: 3.3 × 105 ♀: 2.4 × 105 | 25.1 ♂: 23.6, ♀: 28.8 | 89.0% ♂: 89.0%, ♀: 88.8% |
Piao | 2021-03-26 | 60 | 26 | 18 | 10 | 8 | 15 (83.3%) | 9 (90%) | 6 (75%) | 2.1 × 105 ♂: 2.5 × 105 ♀: 1.8 × 105 | 29.3 ♂: 25.4, ♀: 33.1 | 87.8% ♂: 90.5%, ♀: 85.3% |
2021-04-05 | 60 | 35 | 31 | 14 | 17 | 24 (77.4%) | 13 (93%) | 11 (65%) | ||||
2021-09-01 | 60 | 18 | 14 | 6 | 8 | 13 (92.9%) | 6 (100%) | 7 (88%) | ||||
2021-09-23 | 38 | 22 | 19 | 5 | 14 | 16 (84.2%) | 5 (100%) | 11 (79%) | ||||
Total | 218 | 101 (46.3%) | 82 | 35 | 47 | 68 (82.9%) | 33 (94.3%) | 35 (74.5%) | ||||
Two breeds | Total ♂♀ | 578 | 391 (67.6%) | 293 | - | - | 244 (83.3%) | - | - | 2.8 × 105 | 26.3 | 88.60% |
Total ♂ | - | - | - | 168 | - | - | 159 (94%) | - | 3.1 × 105 | 24.0 | 89.30% | |
Total ♀ | - | - | - | - | 125 | - | - | 88 (68.8%) | 2.2 × 105 | 30.6 | 87.40% | |
