Table 3 Efficiency of producing donor PGC-derived chicks from recipient males by mating with WT hens (Progeny Test I)

From: PGC-based cryobanking, regeneration through germline chimera mating, and CRISPR/Cas9-mediated TYRP1 modification in indigenous Chinese chickens

Recipient males (Breed/Population)

Donor PGCs

No. of microinjected PGCs (male/female)

No. of mated hens (WT)

Mating period (days)

Set of eggs

Hatched chicks

Number of donor PGC-derived offspring (%)

Breed (PGC line)

Period of cryopresavation

#2594♂ (Chahua)

Piao (♂#P28)

Piao (♀#P52)

3 months

~2200/ ~ 3600

4

27

60

44

5 (1.14%)

#2610♂ (Chahua)

~7500/ ~ 5500

6

104

135

91

73 (80.2%)

#2616♂ (Chahua)

~7500/ ~ 5500

6

121

145

91

68 (74.7%)

#2628♂ (Chahua)

~8000/ ~ 7000

5

109

150

123

23 (18.7%)

#2629♂ (Chahua)

~8000/ ~ 7000

5

109

127

107

20 (18.7%)

#2630♂ (Chahua)

~9100/ ~ 5900

4

20

40

34

1 (0.3%)

#2632♂ (Chahua)

~9100/ ~ 5900

5

55

68

58

20 (34.5%)

#2643♂ (Chahua)

~8300/ ~ 7700

4

27

32

23

0

#2644♂ (Chahua)

~8300/ ~ 7700

5

55

69

59

13 (22.3%)

#2588♂ (PCM)

Chahua (♂#CA10-TYRP1-KO)

Not done

~3500/-

4

36

86

65

0

#2595♂ (PCM)

~3500/-

4

170

127

101

4 (0.4%)

  1. The two recipient males (#2610, #2616) that exhibited high efficiency in producing chicks derived from donor PGCs were subsequently used in mating experiments with recipient females in Progeny Test II. The bold letters indicate the high-performing recipient males that were selected for Progeny Test II, as well as the numbers and ratio of PGC-derived offspring from the males.
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