Table 4 Mating experiment among male and female recipient chickens (Progeny Test II)

From: PGC-based cryobanking, regeneration through germline chimera mating, and CRISPR/Cas9-mediated TYRP1 modification in indigenous Chinese chickens

Recipient Chahua females

Donor Piao PGCs

Recipient Chahau males (D-loop haplotypes)

No. of hatched chicks

No. of recipient- derived chicks (%)

No. of donor PGC-derived chicks (%)

ID no.

mtDNA D-loop (haplotypes)

Breed (lines)

D-loop haplotypes

Total (%)

♂ PGC-derived (%)

♀ PGC-derived (%)

♂♀ PGC-derived (Piao) (%)

#2592♀

C

Piao (#P28♂)

Piao (#P52♀)

#P28♂: A

#P52♀: B

#2610♂(C) + #2616♂(C)

10

1 (10.0%)

9 (90.0%)

8 (80.0%)

1 (10.0%)

0

#2599♀

C

#2616♂

27

10 (37.0%)

17 (63.0%)

13 (48.2%)

1 (3.7%)

3 (11.1%)

#2609♀

D

#2610♂

15

5 (33.3%)

10 (66.7%)

8 (53.4%)

2 (13.3%)

0

#2613♀

D

#2610♂ + #2616♂

31

15 (48.4%)

16 (51.6%)

16 (51.6%)

0

0

#2631♀

D

#2610♂ + #2616♂

34

5 (14.7%)

29 (85.3%)

15 (44.1%)

3 (8.8%)

11 (32.4%)

#2633♀

E

#2616♂

17

4 (23.5%)

13 (76.5%)

9 (53.0%)

1 (5.9%)

3 (17.6%)

    

 Total

134

40 (29.9%)

94 (70.1%)

69 (51.4%)

8 (6.0%)

17 (12.7%)

  1. The total number of hatched chicks and the total numbers and ratio of the three categorized donor PGC-derived offspring are indicated by bold letters, respectively.
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