Fig. 3: A fluorescence-based assay for LdtMt2 transpeptidase activity. | Communications Biology

Fig. 3: A fluorescence-based assay for LdtMt2 transpeptidase activity.

From: Biochemical and crystallographic studies of l,d-transpeptidase 2 from Mycobacterium tuberculosis with its natural monomer substrate

Fig. 3

A Principle of the LdtMt2 transpeptidase assay, employing d-amino acid oxidase (DAAO) and horseradish peroxidase (HRP) to report on the release of d-Ala. B Correlation between pIC50 values obtained with the transpeptidase activity assay and fluorogenic thiol probe assay26. Individual data points are shown. C Michaelis-Menten graph of LdtMt2 in reaction with substrate 1. D Michaelis-Menten graph of LdtMt2 in reaction with substrate 3.

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