Table 1 Primers used in the site-directed mutagenesis to achieve the C354S mutation of LdtMt2

From: Biochemical and crystallographic studies of l,d-transpeptidase 2 from Mycobacterium tuberculosis with its natural monomer substrate

Primer

Sequence

LdtMt2C354S-forward

CAGCCATGGTAGTCTGAATGTTAGCCCGAGCAATG

LdtMt2C354S-reverse

CTAACATTCAGACTACCATGGCTGGTATTGGTATG

  1. In bold is the nucleic acid that is changed from the wild type.
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