Table 2 Master mixture for the DAAO assay

From: Biochemical and crystallographic studies of l,d-transpeptidase 2 from Mycobacterium tuberculosis with its natural monomer substrate

Component

Concentration (1.8x)

Final concentration

DAAO

3.6 U/mL

2.0 U/mL

FAD

72 µM

40 µM

HRP

3.6 U/mL

2.0 U/mL

Amplex red

18 µM

10 µM

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