Fig. 3: Impact of PHD2 on mESCs contribution to mesendoderm by EiTix-embryoid formation assays.

a The schematic diagram showing the overall design of embryoid formation with different mESC lines. b The immunostaining against T on the embryoids formed with the WT (n = 4), PHD2-KO (n = 4), and PHD2-OE mESCs (n = 4), respectively. c The schematic diagram showing the overall design of embryoid formation with a mixture of the WT and PHD2-KO mESCs that were labeled with GFP and mCherry, respectively. d The immunostaining against GFP and mCherry on the embryoids (n = 6); The dashed line represents the exclusion of extraembryonic endoderm tissue from the whole embryo. Scale bars, 100 μm; BF bright field, A anterior, P posterior, WT wild type, KO PHD2-KO, OE PHD2-OE, iCdx2 inducible CDX2 expression, iGata4 inducible GATA4 expression.