Fig. 6: Chemogenetic interventions influence feedforward inhibition within MD-ACC pathway. | Communications Biology

Fig. 6: Chemogenetic interventions influence feedforward inhibition within MD-ACC pathway.

From: Deconstruction the feedforward inhibition changes in the layer III of anterior cingulate cortex after peripheral nerve injury

Fig. 6

A Schematic diagram of experimental design. B The whole-cell patch recording of ACC L3 pyramidal neurons. C Representative sEPSC traces recorded from ACC L3 pyramidal neurons. D Representative sIPSC traces recorded from ACC L3 pyramidal neurons. E Quantification of frequency of sEPSCs and sIPSC of ACC L3 pyramidal neurons (Two-way ANOVA, Interaction, F(2, 44) = 4.331, P = 0.0192; sEPSC, ACSF vs. CNO, P = 0.0054; ACSF vs. CNQX, P < 0.0001, CNO vs. CNQX, P < 0.0001; sIPSC, ACSF vs. CNO, P = 0.0469; ACSF vs. CNQX, P = 0.1789, CNO vs. CNQX, P = 0.0285; n = 14 neurons of sEPSC from 6 mice, n = 10 neurons of sIPSC from 5 mice). All data were mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. F Quantification of amplitude of sEPSCs and sIPSC of ACC L3 pyramidal neurons (Two-way ANOVA, Interaction, F(2, 44) = 49.53, P < 0.0001; sEPSC, ACSF vs. CNO, P = 0.9041; ACSF vs. CNQX, P < 0.0001, CNO vs. CNQX, P < 0.0001; sIPSC, ACSF vs. CNO, P = 0.0086; ACSF vs. CNQX, P < 0.0001, CNO vs. CNQX, P = 0.0744; n = 14 neurons of sEPSC from 6 mice, n = 10 neurons of sIPSC from 5 mice). All data were mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.

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