Fig. 4: The radial distribution function demonstrates region-specific T-cell interactions with CD14+ cells in whole slide images.

a RDFs for sample Mel-512 with T-cells as the reference cell demonstrate that intratumor T-cells colocalize with both loaded CD14+ cells (cyan curve) and unloaded CD14+ cells (blue) above the expected distribution (dashed lines). Conversely, intratumor T-cells contact melanoma cells below null expectations (green). b The cohort distributions of ΔCDF demonstrate significant intratumor T-cell colocalization with loaded CD14+ cells, unloaded CD14+ cells, and other T-cells but not tumor cells. ΔCDF is significantly greater for loaded CD14+ cells compared to both unloaded CD14+ cells and melanoma cells. c In the stroma, T-cells colocalize with unloaded CD14+ cells (blue, right) but no colocalization with loaded CD14+ cells. d Cohort wide ΔCDF demonstrates significant intratumor T-cell colocalization with unloaded CD14+ cells and other T-cells but not loaded CD14+ cells. ΔCDF for stromal T-cells is significantly greater for unloaded CD14+ cells compared to loaded CD14+ cells. T-cells similarly show strongly positive ΔCDF. #: 1-sample t-test p < 0.05. *Wilcoxon rank-sum test p < 0.05.