Fig. 1: Transcription and protein levels of CadC under acid-induced conditions. | Communications Biology

Fig. 1: Transcription and protein levels of CadC under acid-induced conditions.

From: Molecular mechanism of proteolytic cleavage-dependent activation of CadC-mediated response to acid in E. coli

Fig. 1

Transcription of cadC in E. coli MG1655 at pH 5.8, 7.0 and 8.0 without (a) or with (b) lysine, detected by qRT-PCR. Data are mean ± SD of n =3 biologically independent replicates. Transcription of cadA (c) and cadB (d) in E. coli MG1655, ΔcadC and ΔcadC-pCL1920cadC strains at pH 5.8 in the presence of lysine, detected by qRT-PCR. Data are mean ± SD of n = 3 biologically independent replicates. Different letters denote significant disparities among various samples at the same detection time, based on Duncan’s test (p < 0.05, n = 3). The protein level of CadC in E. coli MG1655 under different pH conditions. Corresponding Western blot results (e) and their gray values (f) are displayed. The full-length CadC (57.8 kDa) and its suspected cleavage fragment are marked in the diagram. DnaK serves as a loading control for calibrating the grayscale values of the corresponding samples.

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