Fig. 3: Interaction and enzymatic cleavage of CadC by DegP and DegQ in vitro. | Communications Biology

Fig. 3: Interaction and enzymatic cleavage of CadC by DegP and DegQ in vitro.

From: Molecular mechanism of proteolytic cleavage-dependent activation of CadC-mediated response to acid in E. coli

Fig. 3

a SDS-PAGE analysis illustrating the cleavage activity of DegP and DegQ on substrate CadC, comparing the effects on CadC R184Q and CadC with cadaverine. CadC fragment indicate the products post-cleavage, with CadC1-184 used for size calibration. Size-exclusion chromatography analysis of complexes formed between CadC and DegP (b) or DegQ (c). d The localization of CadC184 in cells was determined by subcellular fractionation experiments.

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