Fig. 5: ER-OMM distance-dependent mitochondrial respiration and ATP production. | Communications Biology

Fig. 5: ER-OMM distance-dependent mitochondrial respiration and ATP production.

From: ER-mitochondria distance is a critical parameter for efficient mitochondrial Ca2+ uptake and oxidative metabolism

Fig. 5

a Representative traces (ER-RFP), illustrating the protocol of high-resolution respirometry for quantification of respiration using Oroboros’ protocol for intact cells; blue line: oxygen concentration, red line: oxygen flux. b Oxygen flux in the routine state (R); in the leakage state (L) after the addition of oligomycin, an inhibitor of ATP synthase; after the addition of FCCP, an uncoupler of oxidative phosphorylation to induce maximum respiratory capacity (E). All data are expressed as specific flux, i.e., oxygen consumption normalized to the sample protein content and after subtraction of non-mitochondrial oxygen flux (ROX). c Reserve respiratory capacity obtained by the subtraction of R from E. d Oxygen consumption linked to ATP production, i.e., oligomycin-sensitive respiration obtained by the subtraction of L from R. Data are expressed as mean ± SEM of fold changes above the control of n = 4 (ER-RFP and 10nm-EML) or n = 3 (ER-RFP and 20nm-EML) independent experiments. Unpaired two-tailed t-test 10nm-EML or 20nm-EML vs ER-RFP. *p-value < 0.05; **p-value < 0.01. e Total cellular ATP. Data are expressed as mean ± SEM of % of control (ER-RFP) of n = 6 independent experiments. One-way ANOVA, Tukey post hoc test, **p-value < 0.01; ****p-value < 0.0001.

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