Fig. 7: Expression of 20nm-EML, but not 10nm-EML rescues mitochondrial Ca2+ uptake in PD astrocytes. | Communications Biology

Fig. 7: Expression of 20nm-EML, but not 10nm-EML rescues mitochondrial Ca2+ uptake in PD astrocytes.

From: ER-mitochondria distance is a critical parameter for efficient mitochondrial Ca2+ uptake and oxidative metabolism

Fig. 7

Representative traces and quantifications of raw (a, b) and baseline-normalized (c, d) ATP (100 µM)-induced Ca2+ transients in Ctr-hiA PD-hiA transfected with ER-mRFP, 10nm-EML and 20nm-EML. Box plot from 44–54 cells from three independent experiments. One-way ANOVA with Tukey post hoc test, ****p-value < 0.0001. e Cartoon illustrating MERCS alterations in hiA from LRRK2G2019S patients (PD) compared with healthy subjects (Ctr). Enlargement of ER-OMM distance with specific reduction of 20 nm MERCS leads to failure of high [Ca2+] hot spots to reach mitochondria. The alterations are rescued by the expression of 20nm-EML but not by 10nm-EML. f Quantification of G-aggregates/monomers ratio of JC-1 by flow cytometry in Ctr-hiA and PD-hiA transfected with ER-EBFP and EBFP-expressing 10nm-EML and 20nm-EML. Data are expressed mean ± SEM of percent of 105 gated cells from three independent experiments. One-way ANOVA, Tukey post hoc test. ****p-value < 0.0001.

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