Fig. 3: Gene expression profiles of the THP-1 cell line after being incorporated in HSEs.
From: Characterization of macrophages associated with human skin models exposed to UV radiation
All the samples were analyzed in triplicate. A Heatmap of the DEGs showing the relative expression levels between integrated THP-1 cells in 3D HSE (HSE.THP1) or THP-1 cells in 2D culture (THP-1) (|log2 FC|> 2 and adjusted p value < 0.05). B Volcano plot showing all mapped DEGs with at least twofold enrichment in the HSE.THP-1 population. The red dots indicate DEGs, with the left and right sides representing downregulated and upregulated genes, respectively. The green dots indicate DEGs whose expression levels did not significantly change. The x axis represents the log2-fold change in normalized gene expression, and the y axis represents the log10 adjusted p value < 0.05. C The top 10 significantly enriched GO terms associated with the HSE.THP1 population were generated. The GO terms are ordered according to the gene ratios. The size of the dots represents the number of DEGs associated with the GO terms, and the color represents the value of the adjusted p value (p.adjust). BP biological process, CC cellular component, MF molecular function. D Bar plots showing the number of DEGs in each of the high-level significant KEGG enrichment pathways of the HSE.THP1 population. The KEGG terms are ordered according to the value of p.adjust, represented by color. E Enrichment plots of up- or downregulated genes related to gene sets identified by GSEA based on the KEGG database. Nominal p value < 0.001.
