Fig. 5: Effect of M2G on immunocompetent HSEs under UVA irradiation.
From: Characterization of macrophages associated with human skin models exposed to UV radiation
A Schematic diagram illustrating the experimental procedure used to test the effect of M2G on UVA irradiation in immunocompetent HSEs. Created with BioRender.com. B Representative images of H&E staining of HSE.THP-1 cells; non-exposure, UVA exposure, UVA exposure with or without M2G (i) and detection of DNA double-strand breaks (DSBs) by γ-H2AX IF staining (magenta) (ii). The cell nuclei were visualized by Hoechst staining (blue). The white dotted lines indicate epidermal‒dermal junctions. Scale bar: 100 µm, epidermis (E), dermis (D). C Quantitation of γ-H2AX+ cells in both the epidermal and dermal layers from (B). The data from each layer are presented as the means ± SEMs. Statistical significance is indicated by *p < 0.05, **p < 0.01, and ns, not significant, compared with the non-UVA exposure group (n = 3 per group). Two-way ANOVA followed by Holm‒ŠÃdák’s multiple comparison test was used.
