Fig. 5: FUT2 promotes CRC cell migration and invasion via YAP/TAZ signaling pathway.

A total of 2910 genes regulated by FUT2 were generated from the TCGA-CRC dataset, and then GO (A) and KEGG (B) pathway enrichment analyses of 2910 genes were performed. Relevant data processing details are provided in the Methods section. C SW480 cells were transfected with FUT2-shRNA, and then western blot analysis of CYR61, CTGF, TAZ, LATS1, p-YAP1, and YAP1 expressions were determined. The relative density of protein bands was quantified, normalized to GAPDH, and fold changes were shown in histograms from three independent experiments. D SW480 cells were transfected with FUT2-shRNA, and then western blot analysis of the intracellular distribution of YAP1 and TAZ expressions were determined. The relative density of protein bands was quantified, normalized to α-tubulin or PCNA, and fold changes were shown in histograms from three independent experiments. SW480 cells were transfected with FUT2-shRNA, YAP1-siRNA (E) or TAZ-siRNA (F), and then western blot analysis of CTGF expressions were determined. The relative density of protein bands was quantified, normalized to GAPDH, and fold changes were shown in histograms from three independent experiments. SW480 and DLD-1 cells were transfected with FUT2-shRNA (G) or FUT2/Lv105 (H). After transfection, the cells were treated with Verteporfin (500 nM) for 24 h, and then transwell assay was performed. The number of migrating/invading cells was quantified via Image J software. Scale bar represents 50 μm. Data shown are mean ± SD; n = 3 samples. Scale bar represents 50 μm. Statistical significance was determined using one-way ANOVA using the Bonferroni post-hoc test, with *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: not significant.