Fig. 7: FUT2 promotes the expression of GLUT1 through YAP1 in CRC cells.

SW480 cells were transfected with YAP1-siRNA (A) or FUT2-shRNA (B), and then GLUT1 mRNA levels were assessed by qRT-PCR. Data shown are mean ± SD; n = 3 samples. C SW480 and DLD-1 cells were transfected with FUT2-shRNA, and then western blot analysis of GLUT1 expressions were determined. The relative density of protein bands was quantified, normalized to GAPDH, and fold changes were shown in histograms from three independent experiments. D SW480 and DLD-1 cells were transfected with FUT2-shRNA, and then intracellular glucose (Glu) levels were assessed with detection kit. Data shown are mean ± SD; n = 3 samples. E SW480 cells were transfected with FUT2-shRNA. After transfection, SW480 cells were cultured in 5 mM or 25 mM glucose media for 48 h, and then intracellular ATP, TG and Glu levels were assessed with detection kits. Data shown are mean ± SD; n = 3 samples. F SW480 cells were transfected with FUT2-shRNA. After transfection, SW480 cells were cultured in 5 mM or 25 mM glucose media for 48 h, and then western blot analysis of FASN was determined. The relative density of protein bands was quantified, normalized to α-tubulin, and fold changes were shown in histograms from three independent experiments. G SW480 cells were cultured in 5 mM or 25 mM glucose media for 48 h, and then the mRNA levels of ACC1, FASN, SCD, SREBP-1, CHREBP, MLX and FUT2 were assessed by qRT-PCR. Data shown are mean ± SD; n = 3 samples. Statistical significance was determined using one-way ANOVA using the Bonferroni post-hoc test, with *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: not significant.